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KMID : 0379119870150010014
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Korean Journal of Mycology
1987 Volume.15 No. 1 p.14 ~ p.18
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Protoplast Isolation and Reversion from Lyophyllum ulmarium
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박용환/Park, Yong Hwan
장권열/유창현/유영부/Chang, Kwon Yawl/You, Chang Hyun/Yoo, Young Bok
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Abstract
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This experiment was undertaken to investigate proper conditions for protoplast formation from Lyophbllum ulmarium. Combination of Novozym 234, β-Glucuronidase and β-D-Glucanase with 0.6 M Sucrose was the most effective for isolation of protoplasts. The optimal reaction time of mycelium with the lytic mixture was 3 hrs in shaking condition at 120 strokes min^(-1). When the mycelium of L. ulmarium was cultured for 6 days on yeast glucose agar medium at 25℃, the formation of protoplasts was effective.
The yeast glucose agar medium stabilized with 0.6 M sucrose was the most effective for reversion of protoplasts.
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